抗体的生物素化标记实验要点：

1. 溴化丙胺太林相关蛋白2抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-STOML2/SLP2 

中文名称   溴化丙胺太林相关蛋白2抗体说明书 

别    名  EPB72 like protein 2; HSPC108; OTTHUMP00000021320; SLP 2; SLP2; SLP-2; Stomatin (EPB72) like 2; Stomatin like 2; Stomatin like protein 2; STOML2; STML2_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep 

产品类型   一抗  

研究领域     心 细胞生物 生长因子和激素

蛋白分子量  predicted molecular weight: 39kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human STOML2/SLP2 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

溴化丙胺太林相关蛋白2抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 STOML2 is a 356 amino acid member of the mec-2 family of proteins. Expressed ubiquitously at low levels, STOML2 is highly expressed in heart, liver and pancreas. STOML2 is localized to the cytoplasm with some distribution on the membrane. STOML2 was first identified as an overexpressed protein in human endometrial adenocarcinoma. Changes in cell growth in samples with different levels of STOML2 indicate that STOML2 could play a role in endometrial tumorigenesis. STOML2 is also thought to play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.

Function : STOML2 is similar in sequence to stomatin. It is a 356 amino acid protein with a calculated molecular mass of 38.5 kDa. STOML2 has 3 potential initiator sites, all sharing the same open reading frame. The STOML2 protein contains the cognate stomatin family consensus sequence, but it lacks the characteristic N-terminal hydrophobic domain and palmitoylation consensus sequence. STOML2 shares greatest sequence homology with stomatin and SLP1 in a region predicted to contain beta sheet and alpha helix structures. Northern blot analysis detected a 1.5 kb STOML2 transcript in all tissues examined, with highest levels in heart, liver, and pancreas. Western blot analysis detected STOML2 at apparent molecular masses of 45.5 kDa or 44.6 kDa in all human and mammalian cell lines and tissues examined, including red blood cells. Some cells also showed a faint band at about 34.3 kDa, which may represent translation from an alternate initiation site.

Subcellular Location : Membrane; peripheral membrane protein. Cytoplasm. Associated with the cytoskeleton.

Tissue Specificity : Ubiquitously expressed at low levels.

Similarity : Belongs to the band 7/mec-2 family.

Database links : UniProtKB/Swiss-Prot: Q9UJZ1.1

抗体的鉴定：

1）溴化丙胺太林相关蛋白2抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

PGC-1 Alpha(peroxisome proliferator-activated receptor- Gamma coactivator 1 Alpha) 过氧化物酶体增殖物激活受体γ辅激活子1α抗原Multi-class antibodies规格： 0.5mg

Anti-phospho-ATP citrate lyase(Ser455) 磷酸化三磷酸腺柠檬酸裂解酶抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-ALK/CD246(Tyr1604) 磷酸化间变型激酶抗体 规格 0.1ml

NDP Kinase/nm23 浓缩液 0.1ml 进口分装

USP16 英文名称： 去泛素酶16抗体 0.1ml

Defensin Beta 2 英文名称： 防御素β2/Defensin β2抗体 0.1ml

Anti-phospho-ATP citrate lyase(Ser455) 磷酸化三磷酸腺柠檬酸裂解酶抗体Multi-class antibodies规格： 0.1ml

Anti-phospho C-Myc(pThr58/pSer62)/FITC 荧光素标记抗磷酸化致癌基因C-Myc抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-Bovine IgM 兔抗IgM (亲和层析纯化)Multi-class antibodies规格： 1mg

生长激素受体抗体 Anti-GHR 0.1ml

Rabbit Anti-chicken IgG/FITC FITC标记的兔抗鸡IgG 0.3ml

FABP4 英文名称： 脂肪细胞型脂肪酸结合蛋白 0.1ml

Rhesus antibody Rh phospho-SLK/FYN (Tyr530) 磷酸化酪氨酸蛋白激酶Fyn/同步原癌基因抗体 规格 0.1ml

Rabbit Anti-Bovine IgM 兔抗IgM (亲和层析纯化)Multi-class antibodies规格： 1mg

S100 alpha 2 英文名称： S100A2抗体 0.2ml

EAAT3 英文名称： 胶质细胞谷酸运载蛋白3抗体(神经/上皮细胞谷酸运载蛋白) 0.1ml

胸腺肽 α-1抗体 Anti-Thymosin α-1 0.1ml

Anti-OVA/HRP 辣根过氧化物酶标记兔抗鸡卵白蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-KSR1 (Ser392) 磷酸化Ras信号转导KSR1蛋白抗体 规格 0.1ml

PACAP-38 腺苷酸环化酶激活肽-38(抗原)Multi-class antibodies规格： 0.5mg

CNE1, 人细胞系

NRK-49F 大鼠正常肾成纤维细胞

FCGR2B Others Human 人 CD32b / FCGR2B 人细胞裂解液 (阳性对照)

豹猫肺成纤维样细胞;LCL3

脑平滑肌细胞Many types of cells包装：5 × 105次方(1ml)

人纤维细胞;HT-1080 大鼠肠平滑肌细胞完全培养基 100mL

615小鼠株;Ca761

IL2RA Others Cynomolgus 食蟹猴 IL2RA 人细胞裂解液 (阳性对照)

CM-M021小鼠*上皮细胞完全培养基100mL

EC97076细胞，人细胞 人神经母细胞瘤细胞,IMR-32细胞 狗肾恶性症细胞;DH82

Caov-3(人乳突状卵巢腺癌细胞) 5×106cells/瓶×2

CD34 Others Human 人 CD34 人细胞裂解液 (阳性对照)

溴化丙胺太林相关蛋白2抗体说明书 CNE1, 人细胞系

NRK-49F 大鼠正常肾成纤维细胞

FCGR2B Others Human 人 CD32b / FCGR2B 人细胞裂解液 (阳性对照)

豹猫肺成纤维样细胞;LCL3

脑平滑肌细胞Many types of cells包装：5 × 105次方(1ml)

人纤维细胞;HT-1080 大鼠肠平滑肌细胞完全培养基 100mL