产品订购信息：
英文名称  Anti-RNF56/CBLB 

中文名称   环指蛋白56规格 

别    名  Cas Br M (murine) ecotropic retroviral transforming sequence b; Casitas B lineage lymphoma b; Casitas B lineage lymphoma proto oncogene b; Cbl b; E3 ubiquitin protein ligase CBL B; RING finger protein 56; RNF56; SH3 binding protein CBL B; Signal transduction protein CBL B.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse 

产品类型   一抗  

研究领域     细胞生物 免疫学 信号转导

蛋白分子量  predicted molecular weight: 108kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RNF56/CBLB 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

环指蛋白56规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 CBLB (Cas Br M (murine) ecotropic retroviral transforming sequence b) has high homology to CBL, the proto-oncogene that induces pre-B cell lymphomas and myeloid leukemias in mice. Like CBL, CBLB contains putative nuclear localization signal, zinc finger, leucine zipper, and proline-rich domains. CBLB associates with FYN, FGR, and PLCG1. Acts an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization. In addition to its role in the prevention of chronic inflammation and autoimmunity, CBLB also has a function in acute lung inflammation. There are 4 isoforms produced by alternative splicing.

Function : E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization. [PATHWAY] Protein modification; protein ubiquitination.

Subunit : Interacts with SH3 domain-containing proteins LCK, CRK and SORBS1. Interacts with LCP2 and ZAP70. May interact with CBL. Interacts with SH3 domain-containing proteins VAV1, FYN, FGR, PLCG1, GRB2, CRKL, PIK3R1 and SH3KBP1/CIN85. Identified in heterotrimeric complexes with SH3KBP1/CIN85, CD2AP and ARHGEF7, where one CBLB peptide binds two copies of the other protein. Interacts with poly-ubiquitinated proteins. Dimerization is required for the binding of poly-ubiquitin, but not for the binding of mono-ubiquitin.

Subcellular Location : Cytoplasmic and Nuclear

Tissue Specificity : Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes (at protein level).

Post-translational modifications : Phosphorylated on tyrosine and serine residues upon TCR or BCR activation, and upon various types of cell stimulation.

Auto-ubiquitinated upon EGF-mediated cell activation or upon T-cell costimulation by CD28; which promotes proteasomal degradation.

Similarity : Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.

Contains 1 RING-type zinc finger.

Contains 1 UBA domain.

Notch1/MOTC(Neurogenic locus notch homolog protein 1) 跨膜受体蛋白Notch-1抗原Multi-class antibodies规格： 0.5mg

Anti-AQP3 水通道蛋白-3抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-ATG4D(Ser467) 磷酸化自噬相关蛋白4D抗体 规格 0.1ml

山羊抗人 IgG(H+L)/HRP 0.1ml 美国Jackson公司分装

VGLL1 英文名称： 转录辅助因子退变样蛋白1抗体 0.2ml

DENND2C 英文名称： MAP激酶激活死亡域蛋白2C抗体 0.2ml

Anti-AQP3 水通道蛋白-3抗体Multi-class antibodies规格： 0.1ml

Anti-P-HP1/FITC 荧光素标记抗异染色质蛋白1磷酸化抗体(果蝇)IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-human IgG 兔抗人IgG (亲和层析纯化)Multi-class antibodies规格： 1mg

病毒糖蛋白抗体 Anti-Glycoprotein [Rubella virus] 0.2ml

Rabbit Anti-Biotin/HRP 辣根过氧化物酶标记的兔抗生物素抗体IgG 0.1ml

FAHD2A 英文名称： 延胡索酰乙酰乙酸水解酶抗体 0.2ml

Rhesus antibody Rh phospho-SHC (Tyr317) 磷酸化SH2结构域转化蛋白1抗体 规格 0.1ml

Rabbit Anti-human IgG 兔抗人IgG (亲和层析纯化)Multi-class antibodies规格： 1mg

S100A4 英文名称： S100A4抗体 0.1ml

ETV3L 英文名称： ETV3L蛋白抗体 0.2ml

鼠抗F1蛋白/耶尔森氏菌荚膜抗原F1单克隆抗体 Mouse Anti-YERSINIA PESTIS Monoclonal Antibody 0.1ml

Anti-TSH/Biotin 生物素化促甲状腺素抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-IKK beta(Ser471) 磷酸化KB抑制蛋白激酶β抗体 规格 0.1ml

TrK A (h-NGFR) (p140- TrK A) 神经生长因子的一种(抗原)Multi-class antibodies规格： 0.5mg

293T, SV40转化的人胚肾上皮细胞系

DCE 鸡胚成纤维细胞

IL24 Others Human 人 IL-24 / Ierleukin-24 人细胞裂解液 (阳性对照)

CM-M093小鼠胎儿真皮成纤维细胞完全培养基100mL

髓核细胞培养基NPCM-prf

EB病毒转化的人B淋巴细胞;KMY0901 人真皮淋巴上皮细胞完全培养基 100mL

CL-0072Daudi(人细胞)5×106cells/瓶×2

SDF2 Others Human 人 SDF2 杆状病毒-昆虫细胞裂解液 (阳性对照)

细胞凋亡蛋白酶-3分析试剂盒CAS

HT-29细胞，人细胞 人胚肾细胞,293细胞 肾系膜细胞Many types of cells包装：5 ×105次方(1ml)

家兔骨髓间充质干细胞;2012083MSC

SFRP4 Others Human 人 sFRP4 人细胞裂解液 (阳性对照)

环指蛋白56规格 293T, SV40转化的人胚肾上皮细胞系

DCE 鸡胚成纤维细胞

IL24 Others Human 人 IL-24 / Ierleukin-24 人细胞裂解液 (阳性对照)

CM-M093小鼠胎儿真皮成纤维细胞完全培养基100mL

髓核细胞培养基NPCM-prf

EB病毒转化的人B淋巴细胞;KMY0901 人真皮淋巴上皮细胞完全培养基 100mL

抗体的生物素化标记实验要点：

1. 环指蛋白56规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）环指蛋白56规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。