磷酸化原癌基因c-Met相关酪氨酸激酶抗体规格 英文名称  Anti-phospho-Ron/MST1R(Ser1349)

中文名称  磷酸化原癌基因c-Met相关酪氨酸激酶抗体规格 

别    名 MST1R(phospho S1349); c met related tyrosine kinase; CD136; CD136 antigen; CDw136; Macrophage stimulating 1 receptor (c met related tyrosine kinase); Macrophage stimulating 1 receptor; Macrophage stimulating protein receptor alpha chain; MACROPHAGE STIMULATING PROTEIN RECEPTOR; Macrophage stimulating protein receptor beta chain; Macrophage-Stimulating 1 Receptor (MST1R); Macrophage-stimulating protein receptor beta chain; MSP receptor; Mst1r; MST1R variant RON30; MST1R variant RON62; p185 RON; p185-Ron; Protein-tyrosine kinase 8; PTK 8; ptk8; PTK8 protein tyrosine kinase 8; Recepteur d’origine nantais (RON); RON; RON protein tyrosine kinase; RON variant E2E3; RON_MOUSE; Soluble RON variant 1; Soluble RON variant 2; Soluble RON variant 3; Soluble RON variant 4; Stem cell derived tyrosine kinase.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Mouse

产品类型   一抗 磷酸化抗体  

研究领域    细胞生物 信号转导 细胞周期蛋白 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 30/117/148kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from mouse MST1R around the phosphorylation site of Ser1349

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化原癌基因c-Met相关酪氨酸激酶抗体规格 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 MST1R/Ron, a HGF Receptor/MET-type protein kinase, mediates the biological activities of macrophage-stimulating protein (MSP), a multifunctional cytokine that regulates cell adhesion, motility, growth, and survival. The protein is a membrane-spanning, disulfide-linked heterodimer, which results from cleavage of a glycosylated precursor into 35-kD (alpha) and 150-kD (beta) subunits. Ligand binding results in tyrosine phosphorylation of the beta chain. In knockout studies, MST1R/RON (-/-) mice failed to survive past the periimplantation period. The MST1R/RON gene has been mapped to 3p21, a region of frequent deletion or mutation in small cell lung and renal carcinoma, and has been implicated in the progression of several epithelial cancers. Ron expression has been documented in many normal human tissues. ESTs have been isolated from several tissue libraries, including normal colon, mouth, prostate, and testis and cancerous colon, prostate, stomach, and uterus.

Function : Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.

Subunit : Heterodimer of an alpha chain and a beta chain which are disulfide linked. Binds PLXNB1. Associates with and is negatively regulated by HYAL2. Interacts when phosphorylated with downstream effectors including PIK3R1, PCLG1, GRB2 and GAB1. Interacts with integrin beta1/ITGB1 in a ligand-independent fashion. Isoform sf-Stk forms covalent heterodimers with friend spleen focus-forming virus (FSFFV) gp55.

Subcellular Location : Membrane; Single-pass type I membrane protein.

Tissue Specificity : Expressed in colon, skin, lung and bone marrow.

Post-translational modifications : Proteolytic processing yields the two subunits.

Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site.

Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation.

Similarity : Belongs to the protein kinase superfamily. Tyr protein kinase family.

Contains 3 IPT/TIG domains.

Contains 1 protein kinase domain.

Contains 1 Sema domain.

Database links : UniProtKB/Swiss-Prot: Q62190.3

实验流程：

全、新、优、品、好四大特点：

磷酸化原癌基因c-Met相关酪氨酸激酶抗体规格 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

正在热销的相关产品：

LH/CG Receptor(Luteinizing Hormone/Choriogonadotropin Receptor) 人促黄体生成素受体抗原Multi-class antibodies规格： 0.5mg

Anti-Agrin 聚集蛋白抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-CaMK2 alpha (Tyr231) 磷酸化钙/钙调素依赖蛋白激酶2α抗体 规格 0.1ml

碱性磷酸酶阻断剂 10ml Sigma原料

VGLUT1/BNP1 英文名称： 脑特异性依赖无机磷转运蛋白抗体 0.2ml

DEGS1 英文名称： 退行性精母细胞同源物1抗体 0.2ml

Anti-Agrin 聚集蛋白抗体Multi-class antibodies规格： 0.2ml

Anti-Oct-4 /FITC 荧光素标记胚胎干细胞关键蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-human IgG/HRP 辣根过氧化物酶标记兔抗人IgGMulti-class antibodies规格： 0.1ml

周期素依赖性激酶8抗体 Anti-CDK8 0.2ml

Rabbit Anti-horse IgM/FITC FITC标记的兔抗马IgM 0.1ml

Factor VII 英文名称： 凝血因子7抗体 0.1ml

Rhesus antibody Rh Plakophilin 1 桥粒斑菲素蛋白1抗体 规格 0.2ml

Rabbit Anti-human IgG/HRP 辣根过氧化物酶标记兔抗人IgGMulti-class antibodies规格： 0.1ml

Stanniocalcin 1 英文名称： 斯钙素抗体 0.1ml

Exportin 1/CRM1 英文名称： 染色体区域稳定蛋白/核输出蛋白1抗体 0.1ml

ZCWCC1抗体 Anti-ZCWCC1 0.2ml

Anti-TSP-1/THBS1/FITC 荧光素标记血小板反应蛋白/凝血酶敏感蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-IKK alpha/beta (Ser176/Ser180) 磷酸化KB抑制蛋白激酶α/β抗体 规格 0.1ml

Trk B 神经生长因子受体的一种(抗原)Multi-class antibodies规格： 0.5mg

RGC-5, 小鼠视网膜神经节细胞

CSSTH1 中华鳖心肌来源细胞

RSPO1 Others Human 人 RSPO1 / R-Spondin-1 (aa 1-146) 人细胞裂解液 (阳性对照)

T-107B中性蛋白酶(含100mL酶解缓冲液)10mL

黑色素细胞生长生长添加物MelGS

EB病毒转化的人B淋巴细胞;Mao 人骨骼肌细胞完全培养基 100mL

CL-0080WB-F344(大鼠肝上皮样干细胞)5×106cells/瓶×2

AKT3 Others Human 人 AKT3 杆状病毒-昆虫细胞裂解液 (阳性对照)

SMCFectagen? 平滑肌细胞转染试剂盒

LoVo细胞，人细胞 HEK293,人胚肾上皮细胞,293A细胞 肾动脉平滑肌细胞Many types of cells包装：5 × 105次方(1ml)

家兔肾细胞;RABK3

SFRP4 Others Mouse 小鼠 sFRP4 人细胞裂解液 (阳性对照)

磷酸化原癌基因c-Met相关酪氨酸激酶抗体规格 RGC-5, 小鼠视网膜神经节细胞

CSSTH1 中华鳖心肌来源细胞

RSPO1 Others Human 人 RSPO1 / R-Spondin-1 (aa 1-146) 人细胞裂解液 (阳性对照)

T-107B中性蛋白酶(含100mL酶解缓冲液)10mL

黑色素细胞生长生长添加物MelGS

EB病毒转化的人B淋巴细胞;Mao 人骨骼肌细胞完全培养基 100mL

技术外包服务:

★磷酸化原癌基因c-Met相关酪氨酸激酶抗体规格 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。