全、新、优、品、好四大特点：

磷酸化复制因子A蛋白2抗体品牌 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

磷酸化复制因子A蛋白2抗体品牌 英文名称  Anti-phospho-RPA32/RPA2(Thr21)

中文名称  磷酸化复制因子A蛋白2抗体品牌 

别    名 RPA32/RPA2(phospho T21); 60S acidic ribosomal protein P1; p32; p34; REPA2; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A; Replication Protein A2 (32kDa); Replication protein A2; Replication protein A2, 32kDa; RF-A protein 2; Rf-A2; RFA; RFA2_HUMAN; RP-A p32; RP-A p34; RP21C; RPA 2; RPA 32; RPA; Rpa2; RPA32; RPA34; RpLP1; RpP2.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow

产品类型   一抗 磷酸化抗体  

研究领域    免疫学 染色质和核信号

蛋白分子量  predicted molecular weight: 29 kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human RPA2 around the phosphorylation site of Thr21

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化复制因子A蛋白2抗体品牌 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Function : Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.

Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Subunit : Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3.

Subcellular Location : Nucleus. Nucleus, PML body. Note=Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.

Post-translational modifications : Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). In response to DNA damage, recruited to DNA-repair nuclear foci, as a hypophosphorylated form. The necessary dephosphorylation step is catalyzed by PP4. Subsequent hyperphosphorylation, catalyzed by ATR, is required for RAD51 recruitment to chromatin and efficient DNA repair. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. Phosphorylation at Thr-21 depends upon RFWD3 presence.

Database links : UniProtKB/Swiss-Prot: P15927.1

实验流程：

正在热销的相关产品：

MMP-26(Matrix metalloproteinase-26) 基质金属蛋白酶-26抗原Multi-class antibodies规格： 0.5mg

Anti-AT2R 紧张素Ⅱ受体抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-Beta-Catenin (Ser675) 磷酸化β 连环素蛋白抗体 规格 0.1ml

终止液 12ml 进口分装

VIP Receptor 1 英文名称： 活性肠肽受体-1抗体 0.1ml

DARC 英文名称： Duffy血型趋化因子受体抗体 0.2ml

Anti-AT2R 紧张素Ⅱ受体抗体Multi-class antibodies规格： 0.2ml

Anti-PIBF/FITC 荧光素标记孕激素诱导阻断因子抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-human k-chain 兔抗人k-链 (亲和层析纯化)Multi-class antibodies规格： 1mg

G蛋白偶合受体激酶2抗体 Anti-GRK2 0.2ml

Rabbit Anti-Avidin/PE-Cy3 PE-Cy3标记的兔抗亲和素 0.1ml

FAM164B 英文名称： FAM164B蛋白抗体 0.2ml

Rhesus antibody Rh Phospho-SEK1/MKK4 (Ser257/Thr261) 磷酸化原活化蛋白激酶激酶4抗体 规格 0.1ml

Rabbit Anti-human k-chain 兔抗人k-链 (亲和层析纯化)Multi-class antibodies规格： 1mg

SASH1 英文名称： 抑制基因PEPE1抗体 0.2ml

ErbB 3 英文名称： 表皮生长因子受体3抗体 0.1ml

过敏毒素C5a(补体C5a)抗体 Anti-C5a 0.1ml

Anti-Phospho-Tuberin/TSC2 (Ser1387)/FITC 荧光素标记磷酸化马铃薯球蛋白(性硬化)抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-HSP70(Tyr525) 磷酸化热休克蛋白70抗体 规格 0.1ml

Tubulin- Beta 微管蛋白(抗原)Multi-class antibodies规格： 0.5mg

H9c2大鼠心肌细胞(ATCC来源)

CatL2 家猫肺成纤维样细胞

FGFR4 Others Human 人 FGFR4 / FGF Receptor 4 人细胞裂解液 (阳性对照)

CL-0078EL4(小鼠细胞)5×106cells/瓶×2

AsoFectagen?星形细胞转染试剂盒

EB病毒转化的人B淋巴细胞(彝族);KM934 人脐平滑肌细胞完全培养基 100mL

CL-0103Hep G2(人细胞)5×106cells/瓶×2

IgG2a Others Mouse 小鼠 IgG2a-Fc 人细胞裂解液 (阳性对照)

小鼠肝窦内皮细胞完全培养基 100mL

RAG小鼠肾腺癌细胞 RAG mice renal adenocarcinoma cells MEM培养基(GIBCO)+10%FBS

LIF Protein Mouse 重组小鼠 LIF 蛋白 (Fc 标签)

RTE(大鼠气管上皮细胞) 5×106cells/瓶×2 A375(人类恶性)

磷酸化复制因子A蛋白2抗体品牌 H9c2大鼠心肌细胞(ATCC来源)

CatL2 家猫肺成纤维样细胞

FGFR4 Others Human 人 FGFR4 / FGF Receptor 4 人细胞裂解液 (阳性对照)

CL-0078EL4(小鼠细胞)5×106cells/瓶×2

AsoFectagen?星形细胞转染试剂盒

EB病毒转化的人B淋巴细胞(彝族);KM934 人脐平滑肌细胞完全培养基 100mL

技术外包服务:

★磷酸化复制因子A蛋白2抗体品牌 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。