技术外包服务:

★磷酸化Rad51抗体规格 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

实验流程：

磷酸化Rad51抗体规格 英文名称  Anti-phospho-RAD51(Tyr315)

中文名称  磷酸化Rad51抗体规格 

别    名 RAD51(phospho Y315); BRCA1/BRCA2 containing complex, subunit 5; BRCC 5; BRCC5; DNA repair protein RAD51 homolog 1; DNA repair protein rhp51; E coli RecA homolog; HGNC:9817; Homolog of E coli RecA; homolog of S cerevisiae RAD51; HRAD51; HsRad51; HsT16930; Rad 51; RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae); RAD51 homolog; RAD51 homolog S. cerevisiae; RAD51 S cerevisiae homolog; RAD51A; RECA; RecA homolog E. coli; RecA like protein; RecA, E. coli, homolog of; recombination protein A.

产品属性：

浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat

产品类型   一抗 磷酸化抗体  

研究领域     免疫学 信号转导 转录调节因子

蛋白分子量  predicted molecular weight: 37kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human RAD51 around the phosphorylation site of Tyr315

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化Rad51抗体规格 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.

Function : Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

Subcellular Location : Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix. Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels.

Tissue Specificity : Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

Post-translational modifications : Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.

DISEASE : Defects in RAD51 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.

Similarity : Belongs to the RecA family. RAD51 subfamily.

Contains 1 HhH domain.

Database links : UniProtKB/Swiss-Prot: Q06609.1

Rad51蛋白对细胞调节有重要作用:作为辅助因子参与DNA修复同源重组,维持正常细胞周期；Rad51蛋白在很多组织中都有不同的存在。在和等组织中表达较高，究其原因有报道称：这是DNA被损伤后细胞的一种反应，而这种反应不足以阻止的发生。

全、新、优、品、好四大特点：

磷酸化Rad51抗体规格 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

正在热销的相关产品：

Integrin alpha V/CD51 peptide 整合素αV抗原Multi-class antibodies规格： 0.5mg

Anti-ADRB2 能受体β2抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-CD151/MER2(Ser30) 磷酸化CD151抗体 规格 0.1ml

单组分ABTS染色液 100ml/500ml 用于ELISA，405nm

VLDL Receptor 英文名称： 极受体抗体 0.1ml

DNAJA2 英文名称： 细胞周期蛋白3抗体 0.2ml

Anti-ADRB2 能受体β2抗体Multi-class antibodies规格： 0.2ml

Anti-Phospho-PIM1(Tyr218) /FITC 荧光素标记磷酸化前病毒整合位点蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-MK IgG 兔抗猴IgG (亲和层析纯化)Multi-class antibodies规格： 1mg

代谢型谷氨酸受体3 Anti-GRM3 0.2ml

Dog IgM/PE-CY5 PE-CY5标记的兔抗犬IgM抗体 0.1ml

FAM154A 英文名称： FAM154A蛋白抗体 0.2ml

Rhesus antibody Rh phospho-SCNN1B(Thr615) 磷酸化上皮通道β2抗体 规格 0.1ml

Rabbit Anti-MK IgG 兔抗猴IgG (亲和层析纯化)Multi-class antibodies规格： 1mg

SEMA4G 英文名称： 跨膜蛋白SEMA4G抗体 0.2ml

ECM1 英文名称： 细胞外基质蛋白1抗体 0.1ml

酶1抗体 Anti-CA I 0.1ml

Goat Anti-human Fibrinogen/HRP 辣根过氧化物酶标记羊抗人纤维蛋白原Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh Phospho-HSL (Ser660) 磷酸化荷尔蒙敏感脂肪酶抗体 规格 0.1ml

CCL1/TCA3/I-309 嗜酸粒细胞趋化蛋白CCL1抗原Multi-class antibodies规格： 0.5mg

MN-c, 小鼠皮质神经元

BHK-21 叙利亚仓鼠肾细胞

DLL1 Others Rat 大鼠 DLL1 / Delta-like 人细胞裂解液 (阳性对照)

CL-0150MDA-MB-231(人癌细胞)5×106cells/瓶×2

人平滑肌细胞裂解物HPSMCL

腺病毒转化的人胚肾细胞;AAV-293 人肾足突细胞完全培养基 100mL

CL-0120HuH-7(人细胞)5×106cells/瓶×2

MAPKAPK3 Others Human 人 MAPKAPK3 杆状病毒-昆虫细胞裂解液 (阳性对照)

人牙周膜成纤维细胞裂解物HPLFL

SW 1088[SW-1088;SW1088]细胞，人脑星型细胞 人细胞,OS-RC-2细胞 气管上皮细胞Many types of cells包装：5 × 105次方(1ml)

兔晶体上皮细胞永生系;N/N1003A(RLE)

MSR1 Others Human 人 MSR1 / SCARA1 / CD204 人细胞裂解液 (阳性对照)

磷酸化Rad51抗体规格 MN-c, 小鼠皮质神经元

BHK-21 叙利亚仓鼠肾细胞

DLL1 Others Rat 大鼠 DLL1 / Delta-like 人细胞裂解液 (阳性对照)

CL-0150MDA-MB-231(人癌细胞)5×106cells/瓶×2

人平滑肌细胞裂解物HPSMCL

腺病毒转化的人胚肾细胞;AAV-293 人肾足突细胞完全培养基 100mL