mTOR相关调控蛋白抗体费用 英文名称  Anti-Raptor

中文名称  mTOR相关调控蛋白抗体费用 

别    名 p150 target of rapamycin (TOR) scaffold protein containing WD repeats; P150 target of rapamycin (TOR)-scaffold protein; Regulatory Associated Protein of mTOR; KIAA1303; RPTOR_HUMAN; Regulatory-associated protein of mTOR; p150 target of rapamycin (TOR)-scaffold protein.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit

产品类型   一抗  

研究领域     免疫学 信号转导 细胞凋亡 转录调节因子

蛋白分子量  predicted molecular weight: 147kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human Raptor

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

mTOR相关调控蛋白抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of Raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

Function : Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals; functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation.

Subunit : Interacts with MTOR (By similarity). Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR and AKT1S1. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Binds directly to 4EBP1 and RPS6KB1 independently of its association with MTOR. Binds preferentially to poorly or non-phosphorylated forms of EIF4EBP1, and this binding is critical to the ability of MTOR to catalyze phosphorylation. Forms a complex with MTOR under both leucine-rich and -poor conditions. Interacts with ULK1 in a nutrient-dependent manner; the interaction is reduced during starvation. Interacts (when phosphorylated by AMPK) with 14-3-3 protein, leading to inhibit its activity.

Subcellular Location : Cytoplasm. Lysosome. Note=Targeting to lysosomes depends on amino acid availability.

Tissue Specificity : Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta.

Post-translational modifications : In response to nutrient limitation, phosphorylated by AMPK; phosphorylation promotes interaction with 14-3-3 proteins, leading to negative regulation of the mTORC1 complex. In response to growth factors, phosphorylated at Ser-719, Ser-721 and Ser-722 by RPS6KA1; phosphorylation positively stimulates mTORC1 activity.

Similarity : Belongs to the WD repeat RAPTOR family.

Contains 7 WD repeats.

Database links : UniProtKB/Swiss-Prot: Q8N122.1

乳类雷帕霉素靶蛋白（mTOR）信号通路是涉及细胞增长、、、和纤维化的重要信号通路，其相关分子Raptor也是目前的研究热点。

实验流程：

全、新、优、品、好四大特点：

mTOR相关调控蛋白抗体费用 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

正在热销的相关产品：

NIT2 (nitrilase family, member 2) NIT2蛋白抗原Multi-class antibodies规格： 0.5mg

Anti-Apollon/BIRC6 Apollon凋亡抑制蛋白抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-ATR/ACTR(Ser428) 磷酸化ATR抗体 规格 0.1ml

山羊抗小鼠 IgG(H+L)/AP 0.1ml 美国Jackson公司分装

VSV-G tag 英文名称： VSV-G tag标签抗体 0.1ml

DLST 英文名称： DLST蛋白抗体 0.2ml

Anti-Apollon/BIRC6 Apollon凋亡抑制蛋白抗体Multi-class antibodies规格： 0.2ml

Anti-Phospho-PDGF Receptor beta (Tyr1009) /FITC 荧光素标记磷酸化血小板源性生长因子受体-B抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-mouse IgG/FITC 荧光素标记兔抗小鼠IgGMulti-class antibodies规格： 0.3ml

胶质细胞谷氨酸运载蛋白1 抗体 Anti-EAAT1/Glast 0.1ml

Rabbit Anti-human IgM/PE-Cy5.5 PE-Cy5.5标记的兔抗人IgM 0.1ml

FGFR3 英文名称： 成纤维细胞生长因子受体3抗体 0.1ml

Rhesus antibody Rh Phospho-TIF1 beta(Ser824) 磷酸化转录中介因子Tif1β抗体 规格 0.1ml

Rabbit Anti-mouse IgG/FITC 荧光素标记兔抗小鼠IgGMulti-class antibodies规格： 0.3ml

Synaptojanin 英文名称： 磷酸肌醇磷酸酶蛋白INPP5G抗体 0.2ml

Estrogen receptor beta 英文名称： 雌激素受体β抗体 0.1ml

组织蛋白酶L抗体 Anti-cath-L/CL 0.1ml

Anti-UCP-2/FITC 荧光素标记线粒体脱偶连蛋白2抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-Histone H3(Thr3) 磷酸化组蛋白H3抗体 规格 0.1ml

CCR-6/CD196 peptide 细胞表面趋化因子受体6抗原Multi-class antibodies规格： 0.5mg

RLFs, 大鼠

3T3? 成纤维细胞

CTSB Others Mouse 小鼠 Cathepsin-B / CTSB 人细胞裂解液 (阳性对照)

CL-025*875(人细胞)5×106cells/瓶×2

人膀胱基质成纤维细胞cDNAHBdSF cDNA

人胚肾细胞(亚系克隆);FIP293 人肾管状上皮细胞完全培养基 100mL

CL-0146LTEP-a-2(人)5×106cells/瓶×2

IL27 Others Human 人 IL27 / Ierleukin-27 CHO细胞裂解液 (阳性对照)

人尿道上皮细胞裂解物HUCL

长白山猪胚胎皮肤成纤维样细胞;201184 *癌细胞，CFPAC-1细胞 Ranca细胞，小鼠细胞

SV40T转化的人胚肾细胞;293T

HA Others H1N1 甲型 H1N1 (A/Puerto Rico/8/1934) 血凝素HA1 (Hemagglutinin) 人细胞裂解液 (阳性对照)

mTOR相关调控蛋白抗体费用 RLFs, 大鼠

3T3? 成纤维细胞

CTSB Others Mouse 小鼠 Cathepsin-B / CTSB 人细胞裂解液 (阳性对照)

CL-025*875(人细胞)5×106cells/瓶×2

人膀胱基质成纤维细胞cDNAHBdSF cDNA

人胚肾细胞(亚系克隆);FIP293 人肾管状上皮细胞完全培养基 100mL

技术外包服务:

★mTOR相关调控蛋白抗体费用 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。