产品订购信息：
英文名称  Anti-Phospho-RSK3 (Thr353/356) 

中文名称   磷酸化核糖体蛋白S6激酶家族RSK3抗体规格 

别    名  p90RSK 2; pp90RSK 3; Ribosomal S6 kinase 3; RSK 3 antibody S6K alpha 2; S6K alpha; 90 kDa ribosomal protein S6 kinase 2; HU 2; MAP kinase activated protein kinase 1c; MAPKAPK1C; RPS6KA2; KS6A2_HUMAN.

浓    度   1mg/1ml

规 格  0.1ml/100μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Cow, Horse, Sheep, Guinea Pig 

产品类型   一抗 磷酸化抗体  

研究领域     细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 83kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RSK3 around the phosphorylation site of Thr353/356 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化核糖体蛋白S6激酶家族RSK3抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 RSK3(Ribosomal S6 kinase 3)is a member of a family of 90-kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine/threonine protein kinases that are activated in response to mitogenic stimuli, including extracellular signal-regulated protein kinases Erk1 and Erk2. Rsk3 is a distinct isoform of p90Rsk that translocates to the cell nucleus, phosphorylates potential nuclear targets and may have a unique upstream activator. Several sites, such as Ser380, Thr359, Ser363 and Thr573, are important for its activation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c Fos and CREB.

Function : Serine/threonine-protein kinase that acts downstream ofERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenicand stress-induced activation of transcription factors, regulatestranslation, and mediates cellular proliferation, survival, anddifferentiation. May function as tumor suppressor in epithelialovarian cancer cells.

Subunit : Forms a complex with either MAPK1/ERK2 or MAPK3/ERK1 inquiescent cells. Transiently dissociates following mitogenicstimulation (By similarity).

Subcellular Location : Nucleus. Cytoplasm.

Tissue Specificity : Widely expressed with higher expression inlung, skeletal muscle, brain, uterus, ovary, thyroid and prostate.

Post-translational modifications : Activated by phosphorylation at Ser-218 by PDPK1.Autophosphorylated on Ser-377, as part of the activation process.May be phosphorylated at Thr-356 and Ser-360 by MAPK1/ERK2 andMAPK3/ERK1 (By similarity).

N-terminal myristoylation results in an activated kinase inthe absence of added growth factors (By similarity).

Similarity : Belongs to the protein kinase superfamily. AGC Ser/Thrprotein kinase family. S6 kinase subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 2 protein kinase domains.

Database links : UniProtKB/Swiss-Prot: Q15349.2

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产品名称 规格 备注

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Rabbit Anti-mouse IgG-Fc 兔抗小鼠IgG-Fc (亲和层析纯化)Multi-class antibodies规格： 1mg

G蛋白偶合受体激酶1抗体 Anti-GRK1 0.2ml

Mouse Anti-Bov IgG/PE-Cy7 PE-Cy7标记的小鼠抗IgG 0.1ml

FRMD3 英文名称： FRMD3蛋白抗体 0.1ml

Rhesus antibody Rh Phospho-SEK1/MKK4 (Thr261) 磷酸化原活化蛋白激酶激酶4抗体 规格 0.1ml

Rabbit Anti-mouse IgG-Fc 兔抗小鼠IgG-Fc (亲和层析纯化)Multi-class antibodies规格： 1mg

SNAPIN 英文名称： 突触相关蛋白25结合蛋白抗体 0.1ml

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RN-s, 大鼠纹状体神经元

SK-OV-3 人卵巢腺癌细胞

ITGA6 & ITGB1 Others Human 人 ITGA6 & ITGB1 Heterodimer 人细胞裂解液 (阳性对照)

CL-0312293ET(人胚肾细胞(SV40T和EBNA1基因修饰细胞))5×106cells/瓶×2

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人上皮样细胞;BEAS-2B 人卵巢上皮细胞完全培养基 100mL

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人主动脉平滑肌细胞裂解物HASMCL

SK-HEP-1细胞，人细胞 人低转移细胞,95-C细胞 脑动脉内皮细胞Many types of cells包装：5 × 105次方(1ml)

兔主动脉平滑肌细胞;CCC-SMC-1

TIMP1 Others Human 人 TIMP-1 / TIMP1 人细胞裂解液 (阳性对照)

磷酸化核糖体蛋白S6激酶家族RSK3抗体规格 RN-s, 大鼠纹状体神经元

SK-OV-3 人卵巢腺癌细胞

ITGA6 & ITGB1 Others Human 人 ITGA6 & ITGB1 Heterodimer 人细胞裂解液 (阳性对照)

CL-0312293ET(人胚肾细胞(SV40T和EBNA1基因修饰细胞))5×106cells/瓶×2

人神经束膜细胞总RNAHPC NA

人上皮样细胞;BEAS-2B 人卵巢上皮细胞完全培养基 100mL

抗体的生物素化标记实验要点：

1. 磷酸化核糖体蛋白S6激酶家族RSK3抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）磷酸化核糖体蛋白S6激酶家族RSK3抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。