产品订购信息：
英文名称  Anti-RAD51C 

中文名称   和易感基因RAD51C抗体说明书 

别    名  tudor domain containing 1; Tdrd1; MTR-1; FLJ21082; CT41.1; TDRD1_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse 

产品类型   一抗  

研究领域     细胞生物 表观遗传学

蛋白分子量  predicted molecular weight: 132kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RAD51C C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

和易感基因RAD51C抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Participates in branch migration and Holliday junction resolution and thus is important for processing HR intermediates late in the DNA repair process. Also has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis.

Function : Plays a central role during spermatogenesis by participating in the repression transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the localization of Piwi proteins to the meiotic nuage. Involved in the piRNA metabolic process by ensuring the entry of correct transcripts into the normal piRNA pool and limiting the entry of cellular transcripts into the piRNA pathway. May act by allowing the recruitment of piRNA biogenesis or loading factors that ensure the correct entry of transcripts and piRNAs into Piwi proteins

Subunit : Found in a mRNP complex, at least composed of TDRD1, TDRD6, TDRD7 and DDX4. Interacts with MAEL. Interacts with PIWIL1, PIWIL2 and PIWIL4 (when methylated on arginine residues)

Subcellular Location : Cytoplasm. Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Also present in chromatoid body.

Tissue Specificity : Expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate.

Similarity : Belongs to the TDRD1 family.

Contains 1 MYND-type zinc finger.

Contains 4 Tudor domains.

IL-12R Beta2/IL12RB2 peptide 白细胞介素-12受体β2抗原Multi-class antibodies规格： 0.5mg

Anti-Acinus Acinus抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-CDKN1B/P27kip1 (Ser178) 磷酸化P27抗体/周期素依赖激酶抑制剂抗体 规格 0.1ml

狗IgG 干粉 10mg/50mg 国产

WDR23 英文名称： WDR23蛋白抗体 0.2ml

DLP1 英文名称： 动力相关蛋白1抗体 0.1ml

Anti-Acinus Acinus抗体Multi-class antibodies规格： 0.2ml

Anti-PACAP/VIP receptor-I /FITC 荧光素标记腺苷酸环化酶激活肽受体-I/活性肠肽-I抗体IgGMulti-class antibodies规格： 0.2ml

Rabbit Anti-pig IgG/Biotin 生物素化兔抗猪IgGMulti-class antibodies规格： 0.3ml

脱羧酶蛋白体36抗体 Anti-CXorf36 0.2ml

Rabbit anti-MG IgG/PE-CY5 PE-CY5标记的兔抗长爪沙鼠IgG 0.1ml

FXYD7 英文名称： FXYD离子转运调节因子7抗体 0.2ml

Rhesus antibody Rh phospho-VEGF receptor 2(Tyr1214) 磷酸化内皮生长因子受体2抗体 规格 0.1ml

Rabbit Anti-pig IgG/Biotin 生物素化兔抗猪IgGMulti-class antibodies规格： 0.3ml

SAMD14 英文名称： SAMD14抗体 0.2ml

ENPP1 英文名称： 核苷酸内焦磷酸酶/磷酸二酯酶1抗体 0.2ml

细胞表面趋化因子受体2A抗体 Anti-CCR-2A 0.1ml

Anti-USP1/FITC 荧光素标记泛素特异性蛋白酶1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-HER3 (Tyr1222) 磷酸化HER3受体抗体 规格 0.1ml

CD2AP (CD2-associated protein) 白细胞分化抗原CD2APMulti-class antibodies规格： 0.5mg

MA, 小鼠星形胶质细胞

Eca-109 人细胞

EREG Others Human 人 Epiregulin / EREG 人细胞裂解液 (阳性对照)

CL-0393NCI-H209(人小细胞细胞)5×106cells/瓶×2

人绒毛间叶成纤维细胞总RNAHVMF NA

人永生化淋巴细胞;CNLMG-B5538LC 人肝动脉平滑肌细胞完全培养基 100mL

CL-0180P388D1(小鼠淋巴样瘤细胞)5×106cells/瓶×2

CD58 Others Human 人 LFA-3 / CD58 人细胞裂解液 (阳性对照)

人间充质干细胞-脂肪裂解物HMSC-ad L

ER-30细胞，人癌细胞 大鼠腺癌细胞系,MADB106细胞 淋巴成纤维细胞Many types of cells包装：5 × 105次方(1ml)

B16-F1(小鼠细胞) 5×106cells/瓶×2

NHEM.f M2 Pellet 正常人表皮黑素细胞团块(少年者)，培养在M2培养液中 > 1 mio.cells 人气管上皮细胞裂解物HTEpiCL

和易感基因RAD51C抗体说明书 MA, 小鼠星形胶质细胞

Eca-109 人细胞

EREG Others Human 人 Epiregulin / EREG 人细胞裂解液 (阳性对照)

CL-0393NCI-H209(人小细胞细胞)5×106cells/瓶×2

人绒毛间叶成纤维细胞总RNAHVMF NA

人永生化淋巴细胞;CNLMG-B5538LC 人肝动脉平滑肌细胞完全培养基 100mL

抗体的生物素化标记实验要点：

1. 和易感基因RAD51C抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）和易感基因RAD51C抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。