中文名称  磷酸化成视网膜细胞瘤蛋白抗体规格 

英文名称  Anti-phospho-Rb/p105-Rb(Ser780)

别    名 Rb(Phospho-Ser780); OSRC; P105 RB; P105RB; PP105; PP110; pRb; RB 1; RB1; RB1 protein; Retinoblastoma 1 (including osteosarcoma); Retinoblastoma 1; Retinoblastoma associated protein; Including osteosarcoma; Osteosarcoma; p105-Rb; Rb; RB_HUMAN; Retinoblastoma suspectibility protein; Retinoblastoma-associated protein; Retinoblastoma related osteosarcoma; Retinoblastoma susceptibility gene.

产品属性：

磷酸化成视网膜细胞瘤蛋白抗体规格 浓    度  1mg/1ml

规 格  0.1ml/100μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow

产品类型   一抗 磷酸化抗体  

研究领域     细胞生物 免疫学 染色质和核信号 表观遗传学

蛋白分子量  predicted molecular weight: 106kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated Synthesised phosphopeptide derived from human Rb around the phosphorylation site of (Ser780)

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化成视网膜细胞瘤蛋白抗体规格 产品应用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Nuclear Marker.

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.

Function : Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. ecruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the epressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Subunit : Interacts with ATAD5. Interacts with PRMT2, CDK1 and CDK2. The hypophosphorylated form interacts with and sequesters the E2F1 transcription factor. Interacts with heterodimeric E2F/DP transcription factor complexes containing TFDP1 and either E2F1, E2F3, E2F4 or E2F5, or TFDP2 and E2F4. The unphosphorylated form interacts with EID1, ARID3B, KDM5A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF, DNMT1, LIN9, LMNA, SUV420H1, SUV420H2, PELP1 and TMPO-alpha. May interact with NDC80. Interacts with GRIP1 and UBR4. Interacts with ARID4A and KDM5B. Interacts with E4F1 and LIMD1. Interacts with SMARCA4/BRG1 AND HDAC1. Interacts with adenovirus E1A protein, HPV E7 protein and SV40 large T antigen. Interacts with PSMA3 and USP4. Interacts (when methylated at Lys-860) with L3MBTL1.

Subcellular Location : Nucleus.

Tissue Specificity : Expressed in the retina.

Post-translational modifications : Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis.

N-terminus is methylated by METTL11A/NTM1. Monomethylated at Lys-860 by SMYD2, promoting interaction with L3MBTL1.

DISEASE : Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ('cat eye') is investigated.

Defects in RB1 are a cause of susceptibility to bladder cancer (BLC) [MIM:109800]. A malignancy originating in tissues of the urinary bladder. It often presents with multiple tumors appearing at different times and at different sites in the bladder. Most bladder cancers are transitional cell carcinomas. They begin in cells that normally make up the inner lining of the bladder. Other types of bladder cancer include squamous cell carcinoma (cancer that begins in thin, flat cells) and adenocarcinoma (cancer that begins in cells that make and release mucus and other fluids). Bladder cancer is a complex disorder with both genetic and environmental influences.

Defects in RB1 are a cause of osteogenic sarcoma (OSRC) [MIM:259500].

Similarity : Belongs to the retinoblastoma protein (RB) family.

Database links : UniProtKB/Swiss-Prot: P06400.2

核标志物

实验流程：

全、新、优、品、好四大特点：

磷酸化成视网膜细胞瘤蛋白抗体规格 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

新：产品更新速度较快，基本上每周都有新产品出现。

优：产品质量好，投诉比较少。

好：我公司具有优质的技术团队，产品一旦售出，实验过程中遇到困难可提供在线技术咨询。使您使用产品时没有任何的后顾之忧。

技术外包服务:

★磷酸化成视网膜细胞瘤蛋白抗体规格 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

正在热销的相关产品：

HSP-90 Alpha(Heat Shock Protein 90 Alpha ) 热休克蛋白90α抗体Multi-class antibodies规格： 0.5mg

Anti-phospho-c-Abl(Tyr204) 磷酸化非受体酪氨酸激酶c-Abl抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-CHEK1 (Ser280) 磷酸化细胞周期检测点激酶1抗体 规格 0.1ml

兔抗猪IgG血清 0.5ml 国产

Wnt11 英文名称： 信号通路Wnt11抗体 0.1ml

Phospho-Doublecortin (Ser128) 英文名称： 磷酸化双皮质素抗体 0.1ml

Anti-phospho-c-Abl(Tyr204) 磷酸化非受体酪氨酸激酶c-Abl抗体Multi-class antibodies规格： 0.1ml

Anti-Plectin/FITC 荧光素标记网蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rat IgG (亲和纯化) 大鼠IgGMulti-class antibodies规格： 1mg

高迁移率族蛋白-1抗体 Anti-HMGB1 0.1ml

Goat Anti-Guinea pig IgG 羊抗豚鼠IgG 1mg

FGF8 英文名称： 成纤维细胞生长因子8抗体 0.1ml

Rhesus antibody Rh Phospho-RelB(Ser573) 磷酸化核转录因子NFKB-RelB蛋白抗体 规格 0.1ml

Rat IgG (亲和纯化) 大鼠IgGMulti-class antibodies规格： 1mg

synaptotagmin-2 英文名称： 突触结合蛋白相关基因2抗体 0.1ml

phospho-MEK1 + MEK2 (Ser222 + Ser226) 英文名称： 磷酸化原活化蛋白激酶1抗体 0.1ml

CD3抗体 Anti-CD3 0.1ml

Anti-VDCC-L Beta/FITC 荧光素标记L-型电压依赖型钙通道β抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-GSK3 alpha(Ser21) 磷酸化葡萄糖合成激酶3α抗体 GSK3α(Phospho-Ser21) 规格 0.1ml

CD62L (ELAM-1/L-Selectin ) L选择素抗原Multi-class antibodies规格： 0.5mg

人脐平滑肌细胞

NCI-H358 人非小细胞细胞

FCER2 Others Mouse 小鼠 FCER2A / CD23 人细胞裂解液 (阳性对照)

LX-2, 人肝星形细胞株

人胚;IMR-90

人脐内皮细胞;HUV-EC-C 人甲状腺滤泡上皮细胞完全培养基 100mL

CL-0224SW579(人甲状腺鳞癌细胞 )5×106cells/瓶×2

PARP1 Others Human 人 PARP-1 / PARP 杆状病毒-昆虫细胞裂解液 (阳性对照)

人淋巴成纤维细胞cDNAHLF cDNA

F9细胞， 人肺泡上皮细胞,HPAEpiC细胞 肝实质细胞Many types of cells包装：5 ×105次方(1ml)

狗肾细胞;Super Tube

THPO Others Mouse 小鼠 Thrombopoietin / THPO / TPO 人细胞裂解液 (阳性对照)

磷酸化成视网膜细胞瘤蛋白抗体规格 人脐平滑肌细胞

NCI-H358 人非小细胞细胞

FCER2 Others Mouse 小鼠 FCER2A / CD23 人细胞裂解液 (阳性对照)

LX-2, 人肝星形细胞株

人胚;IMR-90

人脐内皮细胞;HUV-EC-C 人甲状腺滤泡上皮细胞完全培养基 100mL