抗体的生物素化标记实验要点：

1. RAM-11抗体费用 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-Rab27a 

中文名称   RAM-11抗体费用 

别    名  RAB27A; GS2; HsT18676; MGC117246; RAB27; RAM; Rab27a; 2210402C08Rik; 2410003M20Rik; 4933437C11Rik; ash; RB27A_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Pig, Cow, Horse, Sheep 

产品类型   一抗  

研究领域    转录调节因子 合成与降解 转运蛋白

蛋白分子量  predicted molecular weight: 25kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RAM-11 C-terminus 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

RAM-11抗体费用 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Summary: The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq].

Function : Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse.

Subunit : Binds SYTL1, SLAC2B, MYRIP, SYTL3, SYTL4 and SYTL5 (By similarity). Binds MLPH and SYTL2. Interacts with UNC13D.

Subcellular Location : Membrane; Lipid-anchor. Melanosome. Late endosome. Lysosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes to endosomal exocytic vesicles.

Tissue Specificity : Found in all the examined tissues except in brain. Low expression was found in thymus, kidney, muscle and placenta. Detected in melanocytes, and in most tumor cell lines examined. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.

DISEASE : Griscelli syndrome 2 (GS2) [MIM:607624]: Rare autosomal recessive disorder that results in pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes. GS2 patients also develop an uncontrolled T-lymphocyte and macrophage activation syndrome, known as hemophagocytic syndrome, leading to death in the absence of bone marrow transplantation. Neurological impairment is present in some patients, likely as a result of hemophagocytic syndrome. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity : Belongs to the small GTPase superfamily. Rab family.

Database links : UniProtKB/Swiss-Prot: P51159.3

抗体的鉴定：

1）RAM-11抗体费用 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

IL-13(Interleukin-13) 白介素13抗原Multi-class antibodies规格： 0.5mg

Anti-phospho-Acinus(Ser1180) 磷酸化腺泡Acinus蛋白抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh phospho-CDKN1B/P27kip1 (Ser10) 磷酸化P27抗体/周期素依赖激酶抑制剂 规格 0.1ml

IgG 干粉 10mg/50mg/100mg 国产

Wnt16 英文名称： 信号通路Wnt16抗体 0.2ml

Delta 4 英文名称： Notch信号通路Delta样配体4抗体 0.1ml

Anti-phospho-Acinus(Ser1180) 磷酸化腺泡Acinus蛋白抗体Multi-class antibodies规格： 0.1ml

Anti-PLAUR/uPAR/FITC 荧光素标记尿激酶型纤溶酶原激活因子受体抗体IgGMulti-class antibodies规格： 0.2ml

Rat IgM (亲和纯化) 大鼠IgMMulti-class antibodies规格： 1mg

血红素氧合酶-1/热休克蛋白-32抗体 Anti-HO-1/HSP32 0.1ml

Goat Anti-rat IgG 羊抗大鼠IgG 1mg

FoxP3 英文名称： 叉头蛋白P3抗体 0.1ml

Rhesus antibody Rh phospho-RelB(Ser552) 磷酸化转录因子RelB蛋白抗体 规格 0.1ml

Rat IgM (亲和纯化) 大鼠IgMMulti-class antibodies规格： 1mg

Somatostatin Receptor 5 英文名称： 生长抑素受体5抗体 0.1ml

phospho-MEK1 (Thr292) 英文名称： 磷酸化原活化蛋白激酶1抗体 0.1ml

CD33抗体(人) Anti-CD33 0.1ml

Anti-VEGF/FITC 荧光素标记VEGF抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-GSK3 Alpha(Ser21) 磷酸化葡萄糖合成激酶-3α抗体 规格 0.1ml

E-Selectin E选择素抗原Multi-class antibodies规格： 0.5mg

人脐动脉内皮细胞

NCI-H345(悬浮) 小细胞

CD83 Others Mouse 小鼠 CD83 / HB15 人细胞裂解液 (阳性对照)

NCI-N87 [N87]人细胞

人胚肾细胞;293 [HEK-293]

人恶性非霍奇金患者的自然杀伤细胞;NK-92MI [NK92MI] 人成纤维细胞完全培养基 100mL

CL-0226SW626(人细胞)5×106cells/瓶×2

FAM19A4 Others Human 人 FAM19A4 / TAFA4 人细胞裂解液 (阳性对照)

人牙龈成纤维细胞cDNAHGF cDNA

猪胚胎传代细胞;ST 子细胞，SiHa细胞 B22细胞，KM小鼠未分化神经瘤株

Sars结构蛋白表达株;293sars181A

HA Others H5N1 甲型 H5N1 血凝素HA1 (Hemagglutinin) 人细胞裂解液 (阳性对照)

RAM-11抗体费用 人脐动脉内皮细胞

NCI-H345(悬浮) 小细胞

CD83 Others Mouse 小鼠 CD83 / HB15 人细胞裂解液 (阳性对照)

NCI-N87 [N87]人细胞

人胚肾细胞;293 [HEK-293]

人恶性非霍奇金患者的自然杀伤细胞;NK-92MI [NK92MI] 人成纤维细胞完全培养基 100mL