抗体的生物素化标记实验要点：

1. 环指蛋白12抗体规格 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-RNF12 

中文名称   环指蛋白12抗体规格 

别    名  RLIM; RING finger LIM domain-binding protein; Ring finger protein 12; RNF12_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep 

产品类型   一抗  

研究领域    细胞生物 免疫学 表观遗传学

蛋白分子量  predicted molecular weight: 69kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RNF12 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

环指蛋白12抗体规格 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 RLIM, also known as RNF12 (RING finger protein 12) or NY-REN-43, is a 624 amino acid RING-H2 zinc finger protein that is involved in protein ubiquitinylation and subsequent degradation. Expressed in a variety of tissues, RLIM binds to the LIM domain of various proteins and functions as a protein ligase that negatively co-regulates LIM homeodomain (LIM-HD) transcription factors. Through its interaction with Sin3A, a component of the histone deacetylase corepressor complex, RLIM is able to recruit the corepressor complex to LIM-HD proteins, thereby inhibiting LIM-HD transcription. In addition to recruiting the deacetylase complex to LIM-HD proteins, RLIM is able to bind to, ubiquinate and subsequently degrade CLIM proteins, which function as positive co-regulators of LIM-HD transcription factors. RLIM contains one RING-type zinc finger and is implicated in renal cell carcinoma.

Function : E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.

Subunit : Interacts with LIM/homeobox factors such as LHX3. Interacts with LDB1, LDB2 and SIN3A (By similarity). Interacts with LIMK1 (By similarity). Interacts (via N-terminus) with TERF1. Interacts (via C-terminus) with ESR1.

Subcellular Location : Cytoplasmic and Nuclear

Tissue Specificity : Expressed in many tissues.

Similarity : Belongs to the RNF12 family.

Contains 1 RING-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q9NVW2.3

抗体的鉴定：

1）环指蛋白12抗体规格 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

IFABP(Intestinal Fatty acid binding protein) 小肠型脂肪酸结合蛋白抗原Multi-class antibodies规格： 0.5mg

Anti-ABP 雄激素结合蛋白抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-CENP-A (Ser7) 磷酸化着丝粒蛋白A 规格 0.1ml

兔抗人IgG- FC 0.5ml 国产

WNT9A 英文名称： 信号通路Wnt9a抗体 0.1ml

DAPK1 英文名称： 凋亡相关蛋白激酶1抗体 0.1ml

Anti-ABP 雄激素结合蛋白抗体Multi-class antibodies规格： 0.2ml

Anti-PTP-1B/FITC 荧光素标记PTP-1B蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Recomb Protein G/FITC 荧光素(FITC)标记重组蛋白-GMulti-class antibodies规格： 0.5ml

粘附分子整合素β1抗体 Anti-ITG-β1/CD29 0.1ml

SOX10 英文名称： 转录因子SOX10抗体 0.2ml

FRAS1 英文名称： 细胞外基质蛋白FRAS1抗体 0.2ml

Rhesus antibody Rh phospho-PPM1A(Ser375) 磷酸化蛋酸酶2C亚型α抗体 规格 0.1ml

Recomb Protein G/FITC 荧光素(FITC)标记重组蛋白-GMulti-class antibodies规格： 0.5ml

Phospho-SRF (Ser103) 英文名称： 磷酸化血清应答因子抗体 0.1ml

EXOC2 英文名称： 胞吐囊复合体蛋白2抗体 0.2ml

CD45RO抗体 Anti-CD45RO 0.1ml

Anti-SV2A/FITC 荧光素标记突触泡蛋白2A抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-Glucocorticoid Receptor (Ser211) 磷酸化糖皮质激素受体抗体 规格 0.1ml

CDK7/Cyclin H/MAT1 周期素依赖性激酶7抗原Multi-class antibodies规格： 0.5mg

THPO Protein Human 重组人 Thrombopoietin / THPO / TPO 蛋白 (His 标签)

MuM-2C 人眼脉络细胞

CD6 Others Mouse 小鼠 CD6 / TP120 人细胞裂解液 (阳性对照)

hFOB 1.19人SV40转染成骨细胞 The hFOB 1.19 SV40 was ansfected io osteoblasts DMEM/F12(1:1)+10% FBS+300μg/ml G418

EB病毒转化的人B淋巴细胞;KMY0927

人胎儿胸腺细胞株;HFT-8810 [HFT8810] 人淋巴内皮细胞完全培养基 100mL

CL-0248ZR-75-30(人癌细胞)5×106cells/瓶×2

OXSR1 Others Human 人 OXSR1 / OSR1 杆状病毒-昆虫细胞裂解液 (阳性对照)

人肾近曲小管上皮细胞cDNAHRPTEpiC cDNA

SHZ-88细胞，大鼠癌细胞 人(转染TK基因的GLC-82细胞),GLC-82-TK细胞 肺泡上皮细胞Many types of cells包装：5 × 105次方(1ml)

犬胸腺细胞;cf2Th

TNFRSF10D Others Human 人 AILR4 / TNFRSF10D / DCR2 / CD264 人细胞裂解液 (阳性对照)

环指蛋白12抗体规格 THPO Protein Human 重组人 Thrombopoietin / THPO / TPO 蛋白 (His 标签)

MuM-2C 人眼脉络细胞

CD6 Others Mouse 小鼠 CD6 / TP120 人细胞裂解液 (阳性对照)

hFOB 1.19人SV40转染成骨细胞 The hFOB 1.19 SV40 was ansfected io osteoblasts DMEM/F12(1:1)+10% FBS+300μg/ml G418

EB病毒转化的人B淋巴细胞;KMY0927

人胎儿胸腺细胞株;HFT-8810 [HFT8810] 人淋巴内皮细胞完全培养基 100mL