技术外包服务:

★环指蛋白130抗体费用 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

实验流程：

环指蛋白130抗体费用 英文名称  Anti-RNF130

中文名称  环指蛋白130抗体费用 

别    名 GOLIATH; 2510042A13Rik; G1 related zinc finger protein; G1rp; G1RZFP; GP antibody MGC117241; MGC138647; MGC99542; R goliath; RING finger protein 130; GOLI_HUMAN.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Cow, Rabbit, Sheep

产品类型   一抗  

研究领域    细胞生物 免疫学

蛋白分子量  predicted molecular weight: 44kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human RNF130/

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

环指蛋白130抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF130 (ring finger protein 130), also known as GP, G1RZFP (G1-related zinc finger protein) or GOLIATH, is a 419 amino acid single-pass type I membrane protein that shares similarity with a Drosophila zinc-finger protein found in mesoderm known as g1. RNF130 contains one PA (protease associated) domain and a single RING-type zinc finger. Implicated in the regulation of growth factor withdrawal-induced apoptosis of myeloid precursor cells, RNF130 is encoded by a gene located on human chromosome 5q35.3 and mouse chromosome 11 B1.3.

Function : May have a role during the programmed cell death of hematopoietic cells (By similarity). Acts as an E3 ubiquitin-protein ligase.

Subcellular Location : Membrane; Single-pass type I membrane protein (Potential). Cytoplasm.

Tissue Specificity : Ubiquitously expressed. Highly expressed in leukocytes. Not expressed in erythroblasts.

Post-translational modifications : In vivo measurements suggest this protein is glycosylated (PubMed:19159218). In contrast, in vitro experiments failed to detect glycosylation (PubMed:16549277).

Similarity : Contains 1 PA (protease associated) domain.

Contains 1 RING-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q86XS8.1

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