产品订购信息：
英文名称  Anti-RNF7/CKBBP1 

中文名称   环指蛋白7抗体品牌 

别    名  CKBBP 1; CKBBP1; CKII beta binding protein 1; CKII beta-binding protein 1; Rbx 2; Rbx2; RBX2_HUMAN; Regulator Of cullins 2; RING box protein 2; Ring finger protein 7; RING-box protein 2; RNF 7; RNF7; ROC 2; ROC2; SAG; Sensitive to apoptosis gene; Sensitive to apoptosis gene protein; Zinc RING finger protein SAG.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Sheep 

产品类型   一抗  

研究领域    细胞生物 信号转导 细胞凋亡 转录调节因子 表观遗传学

蛋白分子量  predicted molecular weight: 13kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RNF7/CKBBP1 (30-75aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

环指蛋白7抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents. Tissue specificity: Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.

Function : Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents.

Subunit : Probable part of SCF complexes, which consist of SKP1, CUL1, RNF7/RBX2 and a F-box protein. Interacts (preferentially) with CUL5. Also interacts (with lower preference) with CUL1, CUL2, CUL3, CUL4A and CUL4B. Interacts with UBE2F. Interacts with CSNK2B, the interaction is not affected by phosphorylation by CK2.

Subcellular Location : Cytoplasm. Nucleus.

Tissue Specificity : Expressed in heart, liver, skeletal muscle and pancreas. At very low levels expressed in brain, placenta and lung.

Post-translational modifications : Phosphorylation by CK2 is required for efficient degradation of NFKBIA and CDKN1B.

Similarity : Belongs to the RING-box family.

Contains 1 RING-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q9UBF6.1

GSR/GRase(glutathione reductase) 谷胱甘肽还原酶抗原Multi-class antibodies规格： 0.5mg

SCARB2 LIMP-2 / SCARB2 / CD36L2 兔单抗 (FITC) Mouse

Rhesus antibody Rh phospho-CREB-1(Ser133) 磷酸化CREB-1抗体 规格 0.1ml

红细胞裂解液(10X) 10ml

ZBT24 英文名称： 锌指蛋白450抗体 0.2ml

Phospho-Dab1 (Tyr198) 英文名称： 磷酸化磷脂蛋白受体Dab1抗体 0.1ml

SCARB2 LIMP-2 / SCARB2 / CD36L2 兔单抗 (FITC) Mouse

Anti-PTN/FITC 荧光素标记多效生长因子抗体IgGMulti-class antibodies规格： 0.2ml

Streptavidin,SA 链霉亲和素Multi-class antibodies规格： 1mg

整合素α4抗体 Anti-ITG-α/ CD49d 0.1ml

名 价 格(RMB)

Fumarate hydratase 英文名称： 富马酸水合酶抗体 0.2ml

Rhesus antibody Rh Phospho-PRAS40 (Thr246) 磷酸化蛋白激酶AKT底物1抗体 规格 0.1ml

Streptavidin,SA 链霉亲和素Multi-class antibodies规格： 1mg

SAMD9 英文名称： SAMD9蛋白抗体 0.2ml

phospho-EphB1 (Tyr928) 英文名称： 磷酸化酪酸蛋白激酶受体B1抗体 0.1ml

细胞凋亡关联酪氨酸激酶抗体 Anti-AATK 0.1ml

Anti-Wnt3a/FITC 荧光素标记信号通路Wnt3a抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh Phospho-FoxO3a (Ser253) 磷酸化叉头蛋白3A抗体 规格 0.1ml

CNP/CNPase ( 2',3'-cyclic-nucleotide 3'-phosphodiesterase) C型尿肽抗原Multi-class antibodies规格： 0.5mg

FLT3LG Protein Mouse 重组小鼠 FLT3L / Flt3 ligand 蛋白 (Fc 标签)

LA795瘤 肺腺癌

LAIR2 Others Human 人 LAIR2 / CD306 人细胞裂解液 (阳性对照)

SK-OV-3人细胞 Human ovarian cancer cell line SK-OV-3 McCoy'*+10% FBS

大鼠肝细胞瘤;H4-II-E-C3

小鼠骨髓间充质肝细胞(MMSC-bm)(5×105) PANC-1, 人*癌细胞 Human

CL-0321BEL-7405(人细胞)5×106cells/瓶×2

MANF Others Mouse 小鼠 MANF / ARMET 人细胞裂解液 (阳性对照)

小鼠肺大内皮细胞完全培养基 100mL

LLC1[LL/2]小鼠Lewis细胞 LLC1[LL/2] mice with Lewis lung cancer cells 1640+10% FBS

IL1B Protein Human 重组人 IL-1 beta / IL1B 蛋白 (pro form, His 标签)

NCI-H716(人结直肠腺癌细胞) 5×106cells/瓶×2 3T3-L1(小鼠脂肪细胞)

环指蛋白7抗体品牌 FLT3LG Protein Mouse 重组小鼠 FLT3L / Flt3 ligand 蛋白 (Fc 标签)

LA795瘤 肺腺癌

LAIR2 Others Human 人 LAIR2 / CD306 人细胞裂解液 (阳性对照)

SK-OV-3人细胞 Human ovarian cancer cell line SK-OV-3 McCoy'*+10% FBS

大鼠肝细胞瘤;H4-II-E-C3

小鼠骨髓间充质肝细胞(MMSC-bm)(5×105) PANC-1, 人*癌细胞 Human

抗体的生物素化标记实验要点：

1. 环指蛋白7抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）环指蛋白7抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。