抗体的生物素化标记实验要点：

1. G蛋白结合蛋白RAB6A抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

产品订购信息：
英文名称  Anti-RAB6A 

中文名称   G蛋白结合蛋白RAB6A抗体品牌 

别    名  GTP binding protein RAB6B; Oncogene RAB6; Rab GTPase; Rab6A variant 3; Rab 6; RAB6; RAB6 member RAS oncogene family; RAB6A member RAS oncogene family; Ras related protein Rab 6A; RCO4 3; RAB6A_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, ,Zebrafish 

产品类型   一抗  

研究领域    信号转导 G蛋白偶联受体 G蛋白信号

蛋白分子量  predicted molecular weight: 23kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RAB6A 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

G蛋白结合蛋白RAB6A抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves, at each stage, the movement of carrier vesicles; a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway. Rab 6, also known as RAB6B, RAB6A or RAB6A, is a 208 amino acid protein that is ubiquitously expressed and is a member of the small GTPase superfamily.

Function : RAB6A belongs to the small GTPase superfamily. It may be involved in protein transport. It is a regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER) and has a low GTPase activity.

Subunit : Interacts with CCDC64; leads to its accumulation in the pericentrosomal region (By similarity). Interacts with SCYL1BP1. Interacts with VSP52 and RABGAP1. Interacts with GCC2 (via its GRIP domain). Interacts with RAB6IP1 (via its RUN 1 domain). Isoform 1 interacts with RAB6KIFL. Isoform 2 does not interact with RAB6KIFL. Isoform 1 interacts with BICD1. Isoform 2 interacts with BICD1. Isoform 1 interacts with BICD2. Isoform 2 interacts with BICD2. Interacts with TMF1. Isoform 1 (GTP-bound) interacts with DYNLRB1; the interaction is direct. Isoform 2 (GDP-bound) interacts with DYNLRB1; the interaction is direct. Interacts with PIFO.

Subcellular Location : Golgi apparatus membrane; Lipid-anchor.

Tissue Specificity : Ubiquitous.

Similarity : Belongs to the small GTPase superfamily. Rab family.

Database links : UniProtKB/Swiss-Prot: P20340.3

抗体的鉴定：

1）G蛋白结合蛋白RAB6A抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。

GDF8/MSTN(growth differntiation factor 8) 生长分化因子8抗原Multi-class antibodies规格： 0.5mg

FCGR1 CD64 / FCGR1 鼠单抗 (PE) Human

Rhesus antibody Rh phospho-DAG1 (Tyr892) 磷酸化肌相关蛋白DAG1抗体 规格 0.1ml

预染蛋白Marker分子量标准(1.7-40kDa) 2x250μl/50次 MBI原装

Zfp91 英文名称： 相关新基因抗体 0.2ml

DCTN3 英文名称： 动力蛋白激活蛋白亚基3抗体 0.2ml

FCGR1 CD64 / FCGR1 鼠单抗 (PE) Human

Anti-tsg101/FITC 荧光素标记tsg101抗体IgGMulti-class antibodies规格： 0.2ml

Thymosin Alpha-1 胸腺肽 α-1 (胸腺肽-Thymosin alpha-1)Multi-class antibodies规格： 0.5mg

内脂素/内脏脂肪素/腹脂素/内肥素 Anti-Visfatin-1/PBEF1 0.2ml

SGK1 英文名称： 糖皮质激素调节激酶1抗体 0.1ml

phospho-EIF4EBP1 (Ser100) 英文名称： 磷酸化eIF4E结合蛋白抗体 0.1ml

Rhesus antibody Rh phospho-IKK gamma (Ser376) 磷酸化KB抑制蛋白激酶γ抗体 规格 0.1ml

Thymosin Alpha-1 胸腺肽 α-1 (胸腺肽-Thymosin alpha-1)Multi-class antibodies规格： 0.5mg

ZBTB42 英文名称： 锌指蛋白925抗体 0.2ml

DTX2 英文名称： 锌离子结合蛋白DTX2抗体 0.2ml

整合素样金属蛋白酶与凝血酶1型(抗体) Anti-ADAM-TS1/METH1 0.1ml

Anti-RAMP-1/RBITC 红色罗丹明标记受体活性调制蛋白1抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-FER/TYK3(Tyr402) 磷酸化酪氨酸蛋白激酶3抗体 规格 0.1ml

MK1/MAPKK1(mitogen activated protein kinase kinase 1) MK1/MAPKK1抗体(N-端)Multi-class antibodies规格： 0.5mg

VEGFC Protein Human 重组人 VEGF-C 蛋白 (His 标签)

JEG-3 人细胞

IFNA13 Others Mouse 小鼠 IFNA13 / Ierferon alpha-13 人细胞裂解液 (阳性对照)

WPMY-1人正常基质永生化细胞 WPMY-1 of normal human prostate somal immortalized cell DMEM+5%FBS

豹猫肌肉成纤维样细胞;LCM4

小鼠条纹神经细胞(MN-s)(1×106) 5-8F, 人高转移细胞系 Human

CL-0353hFOB 1.19(SV40转染人成骨细胞)5×106cells/瓶×2

KDR Others Mouse 小鼠 VEGFR2 / Flk-1 / CD309 / KDR 人细胞裂解液 (阳性对照)

小鼠肺大平滑肌细胞完全培养基 100mL

CAL 27人舌鳞癌细胞 Human tongue squamous cell carcinoma cell line CAL 27 DMEM+10% FBS

IL36B Protein Human 重组人 IL36B / IL1F8 蛋白

NCI-H446(人小细胞细胞) 5×106cells/瓶×2 COLO 205(细胞)

G蛋白结合蛋白RAB6A抗体品牌 VEGFC Protein Human 重组人 VEGF-C 蛋白 (His 标签)

JEG-3 人细胞

IFNA13 Others Mouse 小鼠 IFNA13 / Ierferon alpha-13 人细胞裂解液 (阳性对照)

WPMY-1人正常基质永生化细胞 WPMY-1 of normal human prostate somal immortalized cell DMEM+5%FBS

豹猫肌肉成纤维样细胞;LCM4

小鼠条纹神经细胞(MN-s)(1×106) 5-8F, 人高转移细胞系 Human