产品订购信息：
英文名称  Anti-RAD50 

中文名称   DNA修复蛋白Rad50抗体说明书 

别    名  DNA repair protein RAD50; hRad50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50_HUMAN; Rad50l; Truncated RAD50 protein.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep 

产品类型   一抗  

研究领域    染色质和核信号 表观遗传学

蛋白分子量  predicted molecular weight: 154kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RAD50 (817-872aa) 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

DNA修复蛋白Rad50抗体说明书 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Function : Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

Subunit : Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis.

Subcellular Location : Nucleus. Chromosome telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.

Tissue Specificity : Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

Post-translational modifications : Phosphorylated upon DNA damage, probably by ATM or ATR

DISEASE : Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.

Similarity : Belongs to the SMC family. RAD50 subfamily.

Contains 1 zinc-hook domain.

Database links : UniProtKB/Swiss-Prot: Q92878.1

FOXM1/HFH 11 peptide 插头蛋白M1抗原Multi-class antibodies规格： 0.5mg

CD27 CD27 / TNFRSF7 兔单抗 (PE) Human

Rhesus antibody Rh phospho-DNA PK/PRKDC(Thr2609) 磷酸化DNA依赖性蛋白激酶抗体 规格 0.1ml

自显影X胶片(蛋白印迹用) 3"×5" Kodak

Zinc finger protein 754 英文名称： 锌指蛋白754抗体 0.1ml

DHX15 英文名称： DHX15蛋白抗体 0.2ml

CD27 CD27 / TNFRSF7 兔单抗 (PE) Human

Anti-RhoA /FITC 荧光素标记RhoA抗体IgGMulti-class antibodies规格： 0.2ml

TP-5(Thymopentin) 胸腺五肽Multi-class antibodies规格： 0.5mg

原活化蛋白激酶激酶1 Anti-MAPKK1 0.1ml

Phospho-SHP2 (Tyr81) 英文名称： 磷酸化蛋白酪酸磷酸酶2抗体 0.1ml

FBRSL1 英文名称： 病毒X蛋白反转录蛋白9抗体 0.2ml

Rhesus antibody Rh Phospho-PIM1(Tyr218) 磷酸化前病毒整合位点蛋白1抗体 规格 0.1ml

TP-5(Thymopentin) 胸腺五肽Multi-class antibodies规格： 0.5mg

ZNF239 英文名称： 锌指蛋白239抗体 0.2ml

DP1 英文名称： 转录因子DP1抗体 0.1ml

芳香烃受体抗体 Anti-AHR 0.1ml

Anti-CD99/E2 antigen/FITC 荧光素标记CD99抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh phospho-FAK(Tyr925) 磷酸化粘着斑激酶抗体 规格 0.1ml

CTBP1(C-terminal binding protein 1) C端结合蛋白1抗原Multi-class antibodies规格： 0.5mg

VEGFA Protein Rat 重组大鼠 VEGF164 / VEGFA 蛋白

HT-29 人细胞

IFNGR1 Others Mouse 小鼠 IFNGR1 / CD119 人细胞裂解液 (阳性对照)

U-118 MG人脑星形胶质母细胞瘤 U-118 MG of brain asocytic blastoma DMEM培养基+10%FBS

家猪皮肤细胞;SSC-S1

大鼠间骨髓间充质干细胞(RMSC-bm)(5×105) MCF-7 [MCF7], 人癌细胞系 Human

CL-0367IM-9(人外周血B淋巴细胞)5×106cells/瓶×2

CD58 Others Human 人 LFA-3 / CD58 人细胞裂解液 (阳性对照)

人平滑肌细胞总RNAHPSMC NA

MDA-MB-231细胞，人癌细胞 BALB/c小鼠ES细胞,B/c-ES细胞 BPH-1, 人细胞

NCI-H1975(人) 5×106cells/瓶×2

HDMEC adult Pellet 人真皮微内皮细胞团块(成年捐献者) > 1 mio.cells 小气道上皮细胞培养基SAEpiCM-prf

DNA修复蛋白Rad50抗体说明书 VEGFA Protein Rat 重组大鼠 VEGF164 / VEGFA 蛋白

HT-29 人细胞

IFNGR1 Others Mouse 小鼠 IFNGR1 / CD119 人细胞裂解液 (阳性对照)

U-118 MG人脑星形胶质母细胞瘤 U-118 MG of brain asocytic blastoma DMEM培养基+10%FBS

家猪皮肤细胞;SSC-S1

大鼠间骨髓间充质干细胞(RMSC-bm)(5×105) MCF-7 [MCF7], 人癌细胞系 Human

抗体的生物素化标记实验要点：

1. DNA修复蛋白Rad50抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）DNA修复蛋白Rad50抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。