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蛋白磷酸酶1结合蛋白/环指蛋白158抗体费用 英文名称  Anti-RNF158

中文名称  蛋白磷酸酶1结合蛋白/环指蛋白158抗体费用 

别    名 Heart protein phosphatase 1-binding protein; HEPP1; Putative E3 ubiquitin protein ligase SH3RF2; Putative E3 ubiquitin-protein ligase SH3RF2; RING finger protein 158; SH3 domain containing RING finger protein 2; SH3 domain-containing RING finger protein 2; SH3R2_HUMAN; Sh3rf2.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit, Sheep

产品类型   一抗  

研究领域    心 细胞生物 细胞周期蛋白 表观遗传学

蛋白分子量  predicted molecular weight: 79kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human RNF158

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

蛋白磷酸酶1结合蛋白/环指蛋白158抗体费用 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. SH3RF2 (SH3 domain containing ring finger 2), also known as RNF158, is a 729 amino acid protein with one RING-type zinc finger domain and three SH3 domains. Via its RING-type zinc finger domain, SH3RF2 binds an E2 ubiquitin-conjugating enzyme. This suggests that SH3RF2 functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and immediately transfers that residue to a protein that is targeted for degradation. Due to alternative splicing events, SH3RF2 is expressed as two different isoforms.

Function : Inhibits PPP1CA phosphatase activity. May be a E3 ubiquitin-protein ligase (Potential). May play a role in cardiac function.

Subunit : Interacts with FASLG and PPP1CA.

Tissue Specificity : Heart (at protein level). Heart and testis. In the heart, present in the apex, left atrium, right atrium, left ventricle and right ventricle, but not in the aorta.

Similarity : Belongs to the SH3RF family.

Contains 1 RING-type zinc finger.

Contains 3 SH3 domains.

Database links : UniProtKB/Swiss-Prot: Q8TEC5.3

实验流程：

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