产品订购信息：
英文名称  Anti-RITA/C12orf52 

中文名称   12号染色体开放阅读框52抗体说明书 

别    名  C12orf52; Chromosome 12 open reading frame 52; RBPJ-interacting and tubulin-associated protein; RITA; RITA_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Cow, Sheep 

产品类型   一抗  

研究领域    细胞生物 发育生物学 神经生物学 信号转导 干细胞 转录调节因子

蛋白分子量  predicted molecular weight: 29kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human RITA/C12orf52 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

12号染色体开放阅读框52抗体说明书 产品应用    ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12 including hypochondrogenesis, achondrogenesis and Kniest dysplasia. Noonan syndrome, which includes heart and facial developmental defects among the primary symptoms, is caused by a mutant form of PTPN11 gene product, SH-PTP2. Chromosome 12 is also home to a homeobox gene cluster which encodes crucial transcription factors for morphogenesis, and the natural killer complex gene cluster encoding C-type lectin proteins which mediate the NK cell response to MHC I interaction. Trisomy 12p leads to facial development defects, seizure disorders and a host of other symptoms varying in severity depending on the extent of mosaicism and is most severe in cases of complete trisomy. The C12orf52 gene product has been provisionally designated C12orf52 pending further characterization.

Function : Tubulin-binding protein that acts as a negative regulator of Notch signaling pathway. Shuttles between the cytoplasm and the nucleus and mediates the nuclear export of RBPJ/RBPSUH, thereby preventing the interaction between RBPJ/RBPSUH and NICD product of Notch proteins (Notch intracellular domain), leading to down-regulate Notch-mediated transcription. May play a role in neurogenesis.

Subunit : Interacts with RBPJ/RBPSUH.

Subcellular Location : Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > centrosome. Shuttles rapidly between the cytoplasm and the nucleus. The function of centrosome localization is still unclear.

Similarity : Belongs to the RITA family.

Database links : UniProtKB/Swiss-Prot: Q96K30.1

Anti- Beta-Amyloid(1-40)/FITC 荧光素标记β淀粉样肽(1-40)抗体IgGMulti-class antibodies规格： 0.2ml

Anti-K3/FITC 荧光素标记抗K3蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Rhesus antibody Rh CXCL13/BCA1 B-淋巴细胞趋化因子抗体 规格 0.1ml

Survivin 细胞凋亡抑制因子4(抗原) 0.5mg

KIRREL1 英文名称： 样蛋白1抗体 0.2ml

Rhesus antibody Rh VSV-G tag VSV-G tag标签抗体 规格 0.1ml

Anti-K3/FITC 荧光素标记抗K3蛋白抗体IgGMulti-class antibodies规格： 0.2ml

Anti-OAT-1/FITC 荧光素标记阴离子转运蛋白-1(抗人)抗体IgGMulti-class antibodies规格： 0.2ml

Goat Anti-Chicken IgG/HRP 辣根过氧化物酶标记羊抗鸡IgGMulti-class antibodies规格： 0.1ml

胞浆-5′-核苷酸酶-Ⅱ抗体 Anti-CD73 0.2ml

Goat Anti-Bovine IgG/Alexa Fluor 488 Alexa Fluor 488标记的羊抗IgG 0.1ml

FLIP delta + gamma 英文名称： 凋亡调节蛋白δ+γ抗体 0.2ml

Rhesus antibody Rh PLC beta 3 磷酯酶Cβ3抗体 规格 0.1ml

Goat Anti-Chicken IgG/HRP 辣根过氧化物酶标记羊抗鸡IgGMulti-class antibodies规格： 0.1ml

DISC1(disrupted in schizophrenia1)(NT) DISC1 N端抗原Multi-class antibodies规格： 0.5mg

Anti-CXCR6 细胞表面趋化因子受体6抗体Multi-class antibodies规格： 0.1ml

Rhesus antibody Rh PBEF (CT) 内脂素/前B细胞克隆增强因子-2(C端抗体) 规格 0.1ml

SELL Kit Mouse 小鼠 CD62L / L-Selectin / SELL ELISA配对抗体 ELISA

TAF12 英文名称： TATA框结合蛋白关联因子12抗体 0.1ml

C3orf30 英文名称： 3号染色体开放阅读框30抗体 0.2ml

Anti-CXCR6 细胞表面趋化因子受体6抗体Multi-class antibodies规格： 0.1ml

改良纤维二糖多粘菌素B多粘菌素E(mCPC)琼脂基础冻干配套试剂SR0260支添加于50ml025纤维二糖多粘菌素B多粘菌素E(mCPC)琼脂基础。

LB Lennox Broth 培养基 250g incubation media LB Lennox Broth 培养基 250g

烟曲霉 支/瓶

桔汁琼脂250g用于饮料中耐酸细菌的分离培养

溶血(0.1%)赫氏琼脂 250g 用于菌分离培养

梭菌属测定用培养基250g用于梭菌属细菌检测

胺蓝-DNA酶琼脂 Toluidine Blue DNAse Agar 用于核糖核酸酶检测

C.L.E.D 培养基 C.L.E.D Medium 250 用于尿液中微生物的选择性分离培养(OXOID方法)

梭菌增菌培养基250g/瓶用于梭菌的增菌培养incubationmedia梭菌增菌培养基250g/瓶用于梭菌的增菌培养

SimmonsCitrateAgar

12号染色体开放阅读框52抗体说明书 BPLS琼脂 250(g)  incubation media BPLS琼脂 250(g)

改良BPLS琼脂 250(g)  incubation media 改良BPLS琼脂 250(g)

XLT4琼脂 250(g)  incubation media XLT4琼脂 250(g)

MLCB琼脂 250(g)  incubation media MLCB琼脂 250(g)

抗体的生物素化标记实验要点：

1. 12号染色体开放阅读框52抗体说明书 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）12号染色体开放阅读框52抗体说明书 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。