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★Rap鸟嘌呤核苷酸交换因子2抗体说明书 分子生物学：质粒抽提、PCR、Q-PCR、RT-PCR、分子生物学：基因合成、引物合成、基因测序、载体构建等

★蛋白工程：原核、哺乳动物蛋白表达系统等

★病毒包装：腺病毒、慢病毒等

★抗体工程：磁珠分选、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★细胞工程：细胞表型分析(凋亡、增殖、周期、迁移、侵袭、修复、克隆形成)、细胞培养、细胞膜制备、稳定细胞株构建、细胞RNAi技术等等。

实验流程：

Rap鸟嘌呤核苷酸交换因子2抗体说明书 英文名称  Anti-RAPGEF2/PDZ-GEF1

中文名称  Rap鸟嘌呤核苷酸交换因子2抗体说明书 

别    名 KIAA0313; Neural RAP guanine nucleotide exchange protein; nRap GEP; NRAPGEP; PDZ domain containing guanine nucleotide exchange factor 1; PDZ domain-containing guanine nucleotide exchange factor 1; PDZ GEF1; PDZ-GEF1; PDZGEF1; RA GEF; RA-GEF; Rap guanine nucleotide exchange factor 2; Rapgef2; RPGF2_HUMAN.

产品属性：

浓    度  1mg/1ml

规 格  0.2ml/200μg

抗体来源  Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep

产品类型   一抗  

研究领域    细胞生物 神经生物学 信号转导 G蛋白偶联受体 交换蛋白 G蛋白信号

蛋白分子量  predicted molecular weight: 167kDa 

性    状   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human RAPGEF2 (501-600aa)

亚    型  IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

Rap鸟嘌呤核苷酸交换因子2抗体说明书 产品应用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 PDZ-GEF1 is a 1,499 amino acid cell membrane protein that functions as a guanine nucleotide exchange factor for Rap 1A, Rap 1B and Rap 2B GTPases. Expressed at highest levels in brain, PDZ-GEF1 is found at low levels in placenta, heart, lung and kidney, and undergoes post-translational phosphorylation following DNA damage. PDZ-GEF1 interacts with MAGI-2 and contains one Ras-GEF domain, a Ras-associating domain, one PDZ (DHR) domain, a single N-terminal Ras-GEF domain and a cyclic nucleotide-binding domain. The gene encoding PDZ-GEF1 maps to human chromosome 4q32.1.

Function : Guanine nucleotide exchange factor (GEF) for Rap1A, Rap1B and Rap2B GTPases. Does not interact with cAMP or cGMP.

Subunit : Interacts with MAGI2.

Subcellular Location : Cell membrane (By similarity). Note=Associated with the synaptic plasma membrane

Tissue Specificity : Highest expression levels in brain. Lower expression levels in heart, kidney, lung, and placenta.

Post-translational modifications : Phosphorylation by PLK2 promotes its activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity : Contains 1 cyclic nucleotide-binding domain.

Contains 1 N-terminal Ras-GEF domain.

Contains 1 PDZ (DHR) domain.

Contains 1 Ras-associating domain.

Contains 1 Ras-GEF domain.

Database links : UniProtKB/Swiss-Prot: Q9Y4G8.1

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Rap鸟嘌呤核苷酸交换因子2抗体说明书 全：公司提供上万种产品，涵盖了生物试剂，elisa试剂盒，标准品，培养基，原装耗材，抗体、培养基、ATCC细胞等，基本上各种科研所需产品在我司都能找到。

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