产品订购信息：
英文名称  Anti-RGMA 

中文名称   反义导向分子RGMA抗体品牌 

别    名  Repulsive guidance molecule A; RGM domain family member A; RGMA; RGMA_HUMAN.

浓    度   1mg/1ml

规 格  0.2ml/200μg

抗体来源   Rabbit

克隆类型   polyclonal

交叉反应   Human, Mouse, Rat, Pig, Cow 

产品类型   一抗  

研究领域    细胞生物 发育生物学 神经生物学 细胞表面分子

蛋白分子量  predicted molecular weight: 28kDa

性    状   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Repulsive Guidance Molecule A 

亚    型   IgG

纯化方法   affinity purified by Protein A

储 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

反义导向分子RGMA抗体品牌 产品应用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 The repulsive guidance molecule (RGM) family of proteins are important in the guidance of growth cones of developing neurons. They are repulsive for a group of axons, those from the temporal half of the retina. RGM have been implicated in both axonal guidance and neural tube closure, but unlike ephrins, semaphorins, netrins and slits, no receptor mechanism for RGM activation has been defined. Dorsal root ganglion axons do not respond to RGM but neogenin (a netrin-binding protein which can function as an RGM receptor) expression can spur RGM responsiveness. The RGM proteins are attached to the membrane by a GPI-anchor. Two members of this family, RGMa and RGMb, are expressed in the nervous system. RGMc, also known as hemojuvelin, is a part of the signaling pathway activating hepcidin and works together with hepcidin to restrict iron absorption in the gut. Defects in the gene encoding for RGMc cause the autosomal recessive disorder juvenile hemochromatosis (JH).

Function : Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

Subunit : Interacts with NEO1, BMP2 and BMP4

Subcellular Location : Cell membrane; Lipid-anchor, GPI-anchor

Similarity : Belongs to the repulsive guidance molecule (RGM) family.

Anti-Tie2/FITC 荧光素标记上皮生长因子样域酪氨酸激酶2IgGMulti-class antibodies规格： 0.2ml

ATF6(activating transcription factor 6) 活化转录因子6抗原Multi-class antibodies规格： 0.5mg

src原癌基因抗体 Anti-SRC 0.2ml

Styk1 英文名称： 丝酸/苏酸/酪酸激酶STYK1抗体 0.2ml

Phospho-Estrogen Receptor alpha (Ser167) 英文名称： 磷酸化雌激素受体ER alpha 抗体 0.1ml

Rhesus antibody Rh phospho-MAP4(Ser1046) 磷酸化微管相关蛋白4抗体 规格 0.1ml

ATF6(activating transcription factor 6) 活化转录因子6抗原Multi-class antibodies规格： 0.5mg

Anti-PARP/FITC 荧光素标记多腺苷二磷酸多聚酶抗体IgGMulti-class antibodies规格： 0.2ml

Goat Anti-human IgA/FITC 荧光素标记羊抗人IgAMulti-class antibodies规格： 0.3ml

人巨细胞病毒PP65/CMV低基质磷脂蛋白抗体 Anti-anti-HCMV PP65/HHV5 PP65 0.1ml

Rabbit Anti-mouse IgG-Fc/PE-CY5 PE-CY5标记的兔抗小鼠IgG-Fc 0.1ml

FMNL1 英文名称： 成蛋白相关蛋白1抗体 0.2ml

Rhesus antibody Rh Phospho-Tuberin/TSC2(Ser1418) 磷酸化性硬化蛋白抗体 规格 0.1ml

Goat Anti-human IgA/FITC 荧光素标记羊抗人IgAMulti-class antibodies规格： 0.3ml

Lymphotactin 小鼠淋巴细胞趋化因子Multi-class antibodies规格： 48T

Anti-FLASH/CASP8AP2 半胱氨酸蛋白酶8相关蛋白2抗体Multi-class antibodies规格： 0.2ml

Rhesus antibody Rh Mouse Anti-Rabbit IgM 小鼠抗兔IgM 规格 1mg

Alpha2-PI (Human Alpha2-plasmin inhititor) ELISA Kit 人α2纤溶酶抑制物 96T

Syntrophin-1+2+3 英文名称： 互养蛋白1,2,3抗体 0.1ml

CCDC6 英文名称： 卷曲螺旋结构域蛋白6抗体(状编码蛋白) 0.2ml

Anti-FLASH/CASP8AP2 半胱氨酸蛋白酶8相关蛋白2抗体Multi-class antibodies规格： 0.2ml

T250g用于霍乱弧菌的培养(SN标准)

K氏培养基 250g 用于果汁或浓缩果汁中脂环芽孢杆菌

EEM培养基 EEM medium 250 用于致病性大肠杆菌的增菌培养和肠杆菌的增菌培养(Japan方法)

Aliz-gal琼脂l/瓶用于大肠菌群快速检测incubationmediaAliz-gal琼脂l/瓶用于大肠菌群快速检测

平板计数琼脂平板(9cm) 10个/包 国际标准平板计数琼脂，含糖，用于细菌总数的测定

Mar

碱性蛋白胨水 Alkaline Peptone Water 用于霍乱弧菌选择性增菌培养(SN标准)

小牛血清 Calf serum 200毫升 诊断试剂用

Schaedler肉汤250g/瓶用于厌氧菌的增菌incubationmediaSchaedler肉汤250g/瓶用于厌氧菌的增菌

pH7.3PBS缓冲液 250g PBS是Tris-HCl缓冲盐溶液

反义导向分子RGMA抗体品牌 m-TGE肉汤 250(g)  incubation media m-TGE肉汤 250(g)

m-TEC琼脂 250(g)  incubation media m-TEC琼脂 250(g)

酵母粉琼脂 250(g)  incubation media 酵母粉琼脂 250(g)

液体沙氏培养基 250(g)  incubation media 液体沙氏培养基 250(g)

抗体的生物素化标记实验要点：

1. 反义导向分子RGMA抗体品牌 如在反应混合液中有叠氮钠或游离氨基存在,会抑制标记反应。因此,蛋白质在反应前要对 0.1mol/L碳酸氢钠缓冲液或0.5mol/L硼酸缓冲液充分透析；

2.所用的NHSB及待生物素化蛋白质之间的分子比按蛋白质表面的ε-氨基的密度会有所不同,选择不当则影响标记的效率,应先用几个不同的分子比来筛选最适条件；

3.用NHSB量过量也是不利的,抗原的结合位点可能因此被封闭,导致抗体失活；

4.由于抗体的氨基不易接近可能造成生物素化不足,此时可加入去污剂如 Triton x-100, Tween20等；

5.当游离ε-氨基(赖氨酸残基的氨基)存在于抗体的抗原结合位点时,或位于酶的催化位点时,生物素化会降低或损伤抗体蛋白的结合力或活性；

6.生物素还可能与不同的功能基团,如羰基、氨基、巯基、异咪唑基及苯酚基,也可与糖基共价结合；

7.交联反应后,应充分透析,否则,残余的生物素会对生物素化抗体与亲和素的结合产生竞争作用；

8.在细胞的荧光标记实验中,中和亲和素的本底低,但由于链霉亲和素含有少量正电荷,故对某些细胞可导致高本底。

抗体的鉴定：

1）反义导向分子RGMA抗体品牌 抗体的效价鉴定：不管是用于诊断还是用于,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。

2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 

如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3）抗体亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。